In the middle and late stage of oilfield exploitation of multi-period superimposed channel tight sandstone oil reservoir in Ansai oilfield, water content of the well is increasing and the effective production of the reservoir is uneven. The breakthrough and water-flooding occur easily during the integral fracturing, which can’t be adapted to the oilfield production situation today. In order to solve this problem, rock mechanics model and triaxial stress field distribution model are built by subdivision and correlation of single sand body between oil and water wells, full-length fracturing numerical simulation are carried out by combining with fracturing parameters design, stress intercalations were formed, which could constrain the distribution of fracturing fracture. Although fractures in the intercalations are formed during fracture operation, gaping fissures are closed with the decrease of pump pressure so that propping agents can’t enter into the intercalations to cause effective fractures, effective production of single sand body in the reservoirs is uneven, and effective producing degree of 60% of layers is relatively low. Thus for the 11 wells which have a low producing degree in the small layer, measures of additional perforation, multiple fracturing, shutting off water and exploitation when separated with other pay zone are proposed, which have an apparent stimulation effect after site implementation. Average daily increase of production is more than 1.2 t. In summary, the technique approach system of subdivision and fracturing treatment of multi-period superimposed channel tight single sand body is formed, which could guide the future development of the similar reservoirs.